Purification and properties of human coagulation factor VII.
نویسندگان
چکیده
منابع مشابه
Purification and Properties of Human Coagulation Factor
Blood coagulation Factor M was purified 100,000fold from fresh frozen human plasma to apparent homogeneity with a yield of 30% based on coagulation assay. The molecular weight estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 48,000. Factor VI1 is composed of a single polypeptide chain with the NHz-terminal sequence Ala-Asn-AlaPhe-Leu-(G1a)-(Gla)-Leu-(Arg)-Pro. It is co...
متن کاملPurification and Characterization of Human Coagulation Factor
We have purified human coagulation Factor V 6,000fold to homogeneity from citrated plasma using polyethylene glycol 6000 precipitation, adsorption of Factor V to barium citrate, DEAE-Sepharose chromatography, and gel filtration on Ultrogel AcA 34 (yield 21%). Human Factor V is a single polypeptide chain before and after disulfide bond reduction with an apparent M, = 335,000 as determined by ele...
متن کاملTissue factor-dependent autoactivation of human blood coagulation factor VII.
We recently showed that single-chain zymogen factor VII is converted to two-chain factor VIIa in an autocatalytic manner following complex formation with either cell-surface or solution-phase relipidated tissue factor apoprotein (Nakagaki, T., Foster, D. C., Berkner, K. L., and Kisiel, W. (1991) Biochemistry 30, 10819-10824). We have now performed a detailed kinetic analysis of the autoactivati...
متن کاملHuman blood coagulation factor XI. Purification, properties, and mechanism of activation by activated factor XII.
Human blood coagulation Factor XI was purified in 20% yield from plasma by ion exchange chromatography. The specific clotting activity of purified Factor XI was 250 & 20 units/mg implying that its concentration in normal titrated human plasma is 4 pg/ml. The purified Factor XI in its precursor form gave a single protein band on polyacrylamide gels in the presence of sodium dodecyl sulfate with ...
متن کاملAcquired deficiency of coagulation factor VII
Factor VII (FVII) is found in small amounts in plasma and has a very short half-life in circulation. FVII is vitamin K-dependently synthesized in the liver. As such, hepatopathies, vitamin K deficiency, or use of vitamin K antagonists is the cause of acquired deficiency. Other types of acquired FVII deficiencies are rare.1 Here we describe a case of acquired factor VII deficiency associated to ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)86020-9